Gene-edited iPSCs (induced Pluripotent Stem Cells) AffiCELL represent a cutting-edge advancement in stem cell technology, enabling precise genetic modifications to enhance research and therapeutic applications. These iPSCs are engineered using state-of-the-art gene-editing techniques to achieve specific genetic alterations.

Components and Functionality

• Gene-Editing Tools: AffiCELL provides access to advanced gene-editing tools such as CRISPR/Cas9, TALENs, and ZFNs (Zinc Finger Nucleases). These technologies are used to introduce targeted mutations, corrections, or insertions into the iPSC genome.
• Delivery Systems: The kits include optimized delivery systems for efficient introduction of gene-editing components into iPSCs. This includes plasmid vectors, viral vectors, and nanoparticles designed to enhance transfection efficiency and minimize off-target effects.
• Selection Markers: AffiCELL kits provide selection markers to facilitate the identification and isolation of successfully edited iPSCs. These markers are used to select cells that have incorporated the desired genetic modifications.

Applications

• Functional Studies: Gene-edited iPSCs allow researchers to investigate the role of specific genes in cellular processes and disease mechanisms. This can lead to insights into gene function and identification of potential therapeutic targets.
• Disease Modeling: Creating disease-specific mutations in iPSCs enables the development of accurate disease models, facilitating the study of genetic diseases and the testing of new treatments.
• Therapeutic Development: Gene-edited iPSCs can be used to develop cell-based therapies with improved efficacy and reduced risk of genetic disorders. They offer potential for personalized medicine by correcting genetic defects in patient-specific cells.

Advantages

• Precision Editing: AffiCELL’s gene-editing kits provide precise control over genetic modifications, allowing for targeted changes with high accuracy.
• Efficiency: Advanced delivery systems and validation assays enhance the efficiency of gene editing and ensure reliable outcomes.
• Versatility: The kits support a wide range of genetic modifications and applications, from basic research to therapeutic development.