iPSC (induced Pluripotent Stem Cell) Characterization Kits AffiCELL are specialized tools designed for the detailed analysis and validation of pluripotent stem cells. These kits provide a comprehensive set of reagents and protocols for assessing the quality, identity, and pluripotency of iPSCs.

Components and Functionality

• Surface Marker Antibodies: The kits include antibodies targeting key cell surface markers such as Tra-1-60, Tra-1-81, SSEA-3, and SSEA-4. These markers are essential for confirming the pluripotent state of iPSCs through flow cytometry or immunocytochemistry.
• Pluripotency Assays: AffiCELL kits provide assays for evaluating pluripotency, including alkaline phosphatase staining and embryoid body formation assays. These assays help confirm that iPSCs retain their ability to differentiate into various cell types.
• Gene Expression Analysis: The kits offer reagents for quantitative PCR (qPCR) and RT-PCR to assess the expression of pluripotency-related genes such as Oct4, Sox2, Nanog, and Klf4. This helps validate the molecular signature of iPSCs.
• Karyotyping: AffiCELL kits include protocols and reagents for chromosomal analysis to ensure the genetic stability of iPSCs. Karyotyping helps detect any chromosomal abnormalities that might affect cell quality.

Applications

• Quality Control: Characterization kits are essential for quality control in research and clinical applications, ensuring that iPSCs meet the necessary criteria for further use.
• Research Validation: These kits help validate the pluripotent nature of iPSCs, supporting the credibility of research findings and experimental outcomes.
• Therapeutic Development: Accurate characterization of iPSCs is crucial for developing safe and effective cell-based therapies.

Advantages

• Comprehensive Analysis: The kits provide a wide range of assays and reagents for thorough characterization, covering multiple aspects of iPSC quality and identity.
• High Sensitivity and Specificity: Advanced reagents and protocols ensure precise and reliable results in assessing pluripotency and cell quality.
• Ease of Use: User-friendly protocols and comprehensive guidelines facilitate efficient and accurate characterization of iPSCs.