IPSCs Differentiation Kits
iPSCs (induced pluripotent stem cells) differentiation kits provide optimized reagents and protocols to guide the transformation of iPSCs into specific, functional cell types. These kits are designed to mimic in vivo developmental pathways, enabling the generation of cells from all three germ layers—ectoderm, mesoderm, and endoderm.
Components of iPSCs Differentiation Kits
- Basal Media:
- Optimized culture media specific to the target lineage.
- Supplements like B27, N2, and small molecules to enhance differentiation efficiency.
- Growth Factors and Cytokines:
- Lineage-specific signaling molecules such as Activin A, BMP4, FGF2, and VEGF.
- Extracellular Matrix (ECM) Coatings:
- Matrix components such as laminin, fibronectin, or vitronectin for cell adhesion and growth.
- Validation Tools:
- Antibodies for lineage markers (e.g., SOX17, PAX6, TnT).
- Functional assay reagents to confirm cell functionality.
Applications
- Regenerative Medicine:
- Differentiated cells for transplantation in diseases like Parkinson’s, diabetes, or myocardial infarction.
- Disease Modeling:
- Generation of patient-specific cell types to study disease mechanisms.
- Drug Discovery and Screening:
- High-throughput screening of drugs on human-relevant cell types.
- Assessment of toxicity and efficacy.
iPSCs differentiation kits streamline the generation of specific cell types from pluripotent cells, enabling high-quality, reproducible results for a variety of research and clinical applications. Their pre-optimized nature makes them indispensable tools for advancing stem cell technology and its applications in science and medicine.
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