Human & Animal Primary Cells

Primary cells are directly isolated from human or animal tissues and closely mimic the physiological state of cells in vivo. Unlike immortalized cell lines, primary cells have a limited lifespan, maintaining their original phenotypic and functional characteristics. They are a critical resource for studying biological mechanisms, drug screening, and translational research due to their relevance to natural cellular processes.

Content on Human & Animal Primary Cells

Characteristics:

• Source: Derived from specific tissues, including blood, skin, lung, liver, and brain.
• Heterogeneity: Retain the diversity of cell types present in the original tissue.
• Limited Proliferation: Exhibit finite division potential governed by the Hayflick limit.
• Specificity: Maintain organ- and species-specific markers and functions.

Cultivation:

• Require specialized growth media enriched with tissue-specific growth factors.
• Strict sterility and adherence to optimal environmental conditions are essential for viability.
• Contamination risks, particularly from mycoplasma, require routine monitoring.

Applications of Human & Animal Primary Cells

• Biomedical Research:
  • Used to study physiological and pathological processes at the cellular level.
  • Enable the investigation of species-specific responses.
• Drug Discovery & Development:
  • Predictive toxicology and pharmacokinetic studies in primary hepatocytes.
  • High-fidelity models for efficacy testing.
• Cancer Research:
  • Tumor-derived primary cells aid in understanding patient-specific cancer behavior.
  • Essential for precision medicine and therapeutic targeting.

Primary cells provide unparalleled physiological relevance, making them indispensable tools for studies requiring accurate biological modeling. Their use underscores the necessity for high-quality isolation and culture techniques to maximize experimental fidelity.